T-Cloning Vector for Plasmid-Based 16S rDNA Analysis
نویسندگان
چکیده
منابع مشابه
Identification of genomic heterogeneity among Lactococcus lactis strains by plasmid profiling, PFGE and 16S rDNA sequence analysis.
Lactococcus lactis strains are used commonly as starters, which contribute to desirable flavour and texture properties known as strain-specific, in dairy industry. Genomic heterogeneity of 30 L. lactis strains originating from Turkey and characterized phenotypically were investigated in this study. Plasmid profiling, PFGE and 16S rDNA sequence analyses were performed to determine the genetic va...
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UNLABELLED Commercial cucumber fermentations are typically carried out in 40000 L fermentation tanks. A secondary fermentation can occur after sugars are consumed that results in the formation of acetic, propionic, and butyric acids, concomitantly with the loss of lactic acid and an increase in pH. Spoilage fermentations can result in significant economic loss for industrial producers. The micr...
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The present study dealt with the identification of a bacterium having dominance in earthworm (Lennogaster pusillus) midden by 16S rDNA based genomic analysis. The bacterium identified was Aeromonas punctata strain JM10 (Genbank Accession No: GU205197.1). Isolated DNA of the bacterium showed amplicon band of 1500 bp, when resolved on agarose gel. Amplification of the 16S rDNA gene from the Aerom...
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MOTIVATION 16S rDNA pyrosequencing is a powerful approach that requires extensive usage of computational methods for delineating microbial compositions. Previously, it was shown that outcomes of studies relying on this approach vastly depend on the choice of pre-processing and clustering algorithms used. However, obtaining insights into the effects and accuracy of these algorithms is challengin...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1999
ISSN: 0736-6205,1940-9818
DOI: 10.2144/99262bm08